The gel buffer ions are Bis-Tris+and Cl(pH 6.4). Edit this message by logging in to your account, Note: Product prices and availability are subject to change without notice, Electrophoresis, Western Blotting and ELISA, Chromatography and Mass Spectrometry Reagents, Chlorine and pH Test Kits and Test Strips, Applied Biosystems™ TaqMan® Assay and Arrays Search Tool, Applied Biosystems™ TaqMan® Custom Assay Design Tools, Applied Biosystems™ Custom qPCR Primers and TaqMan® Probes Tool.  MES or MOPS () serve as the trailing ion in the running buffer. 1D Gel Electrophoresis, Protein Gel Electrophoresis, Proteins, Expression, Isolation and Analysis, Western Blotting. However, we are committed to improving your shopping experience. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. NuPAGE Antioxidant may be used with NuPAGE Transfer Buffer to enhance transfer of reduced proteins to membranes. Remove the comb, and rinse the gel wells three times using 1X Running Buffer. It maintains the neutral pH environment established during electrophoresis. For reduced samples, add 1 mL of NuPAGE™ Antioxidant to 400 mL 1X SDS Running Buffer. Use to transfer proteins from NuPAGE Novex gels to membranes for Western blotting. NuPAGE Transfer Buffer can be used with the Novex Semi-Dry Blotter for semi-dry transfer or with the XCell II™ Blot Module for wet (tank) transfer. This makes the NuPAGEfiLDS Sample Buffer more viscous and difficult to pipet as compared … The neutral pH protects against modification of amino acid side chains and is compatible with N-terminal protein sequencing using Edman degradation. Please sign in to view account pricing and product availability. Fisher Scientific is always working to improve our content for you. Description. Description NuPAGE Transfer Buffer can be used with the Novex Semi-Dry Blotter for semi-dry transfer or with the XCell II™ Blot Module for wet (tank) transfer. endstream endobj 94 0 obj <>/ExtGState<>>>/Subtype/Form>>stream You can also view your contract price by searching for this item(s) on GSA Advantage. Privacy Policy. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. b. 3 Prepare gel a. 1D Gel Electrophoresis, Protein Gel Electrophoresis, Proteins, Expression, Isolation and Analysis, Western Blotting. Your feedback has been submitted. The NuPAGEfiLDS Sample Buffer is a 4X concentrated solution containing twice as much LDS as the 2X concentration of NovexfiTris-Glycine SDS or Tricine SDS Sample Buffer. Remove the white tape near the bottom of the gel cassettes. We will not share your information for any other purposes. The unique WedgeWell design of the Bolt Bis-Tris Plus gel allows loading of up to 2X more sample volume than other precast gels. 1D Gel Electrophoresis, Protein Gel Electrophoresis, Proteins, Expression, Isolation and Analysis, Western Blotting. By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. NuPAGE Antioxidant may be used with NuPAGE Transfer Buffer to enhance transfer of reduced proteins to membranes. The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. NuPAGE Transfer Buffer can be used with the Novex Semi-Dry Blotter for semi-dry transfer or with the XCell II™ Blot Module for wet (tank) transfer. NuPAGE Transfer Buffer (20X) is used to transfer proteins from NuPAGE Bis-Tris and NuPAGE Tris-Acetate gels to membranes for western blotting. NuPAGE Antioxidant may be used with NuPAGE Transfer Buffer to enhance transfer of reduced proteins to membranes. NuPAGE Transfer Buffer can be used with the Novex Semi-Dry Blotter for semi-dry transfer or with the XCell II™ Blot Module for wet (tank) transfer. For Research Use Only. Description. This product(s) resides on a Fisher Scientific GSA or VA contract. H‰Òw6PH.V0 C…âä. NuPAGE and Bolt gels are a good choice before downstream transfer and sensitive western blot analysis and any other technique where protein integrity is crucial. The NuPAGE®Bis-Tris discontinuous buffer system involves three ions: Chloride () from the gel buffer and serves as a leading ion due to its high affinity to the anode relative to other anions in the system. Not for use in diagnostic procedures. NuPAGE®Transfer Buffer (20X) 50 mL 50 mL NuPAGE®Antioxidant 1 mL -- Methanol* 100 mL 100 mL Deionized Water 849 mL 850 mL Total Volume 1,000 mL 1,000 mL *NuPAGE®Transfer Buffer with 10% methanol provides optimal transfer of a single gel in the blot module. Please use the form below to provide feedback related to the content on this product. NuPAGE Antioxidant may be used with NuPAGE Transfer Buffer to enhance transfer of reduced proteins to membranes. Description NuPAGE Transfer Buffer can be used with the Novex Semi-Dry Blotter for semi-dry transfer or with the XCell II™ Blot Module for wet (tank) transfer. These products typically do not have pictures or detailed descriptions. To place an order, contact Fisher Scientific Customer Service. Prepare buffers Add 50 mL of 20X NuPAGE™ MES or MOPS SDS Running Buffer to 950 mL of deionized water to prepare 1X SDS Running Buffer. All contact information provided shall also be maintained in accordance with our We appreciate your feedback. LÉ´4 S S6måã°­‰Jİ�ÏKvt™:3)«7ßKl¢©àËf5Øòÿ ““ NuPAGE Antioxidant may be used with NuPAGE Transfer Buffer to enhance transfer of reduced proteins to membranes.