Bio-Rad’s high-quality premixed electrophoresis running buffers are used for. Using the Certificate of Analysis search tool, you can enter the catalog number and lot/control number for the specific product you have on hand and download the exact Certificate of Analysis you need. Certificates of Analysis are associated not only with a product, but also with a specific lot of that product. MES (0.5 M, pH 6) preparation guide and recipe. 0.03 g of bromophenol blue 1.2 mL of 1 M Tris-HCl pH 6.8 Recipe can be automatically scaled by entering desired final volume. Yes. Make 5 mL aliquots, store at -20°C If you are an educator at the K–12 or college level, contact your local Customer Service office to establish an education account number. Final volume 1 L. SDS-PAGE marker buffer 4.8 mL of H2O $(document).ready(function() { var region = $("#headlangID .lang strong").html(); $(".footer-nav > a:nth-child(3)").after(" | Web Accessibility");});Trademarks | Filter and degas, SDS-PAGE gel making buffer Autoclave, 10X PBS (1 L) No need to pH, filter, or degas These discounted products are indicated by an EDU suffix (for example, 1660003EDU). To receive the latest news, promotions, and more, sign up for Bio-Rad updates by entering your email address below. Privacy$(document).ready(function () {$(".links2").append('');});© 2020 Bio-Rad Laboratories, Inc. Terms & Conditions | Make 1 mL aliquots, store at -20°C It has minimal UV absorbance and is often used as a running buffer for bis-tris gels. Dissolve 30.0 g of Tris base, 144.0 g of glycine, and 10.0 g of SDS in 1000 ml of H 2 O. be applied if it is lower than the 1.5 M Tris-HCl (for separating gel) $(document).ready(function(){$('.helpemail').remove();$('.countrylist td a:contains("Brazil")').text('Brasil');$('.countrylist td a:contains("Mexico")').text('México');$('.countrylist td a:contains("Austria")').text('Österreich');$('.countrylist td a:contains("Belgium")').text('België/Belgique/Belgien');$('.countrylist td a:contains("Denmark")').text('Danmark');$('.countrylist td a:contains("Finland")').text('Suomi');$('.countrylist td a:contains("Germany")').text('Deutschland');$('.countrylist td a:contains("Italy")').text('Italia');$('.countrylist td a:contains("Norway")').text('Norge');$('.countrylist td a:contains("Russian Federation")').text('Россия');$('.countrylist td a:contains("Spain")').text('España');$('.countrylist td a:contains("Sweden")').text('Sverige');$('.countrylist td a:contains("Switzerland")').text('Schweiz/Suisse');$('.countrylist td a:contains("The Netherlands")').text('Nederland');$('.countrylist td a:contains("Japan")').text('日本');$('.countrylist td a:contains("China")').text('中国');$('.countrylist td a:contains("Korea")').text('한국');});Please select your location to view the products, information, and services available, including news, promotions and events.. Premixed buffers are available for a variety of protein and nucleic acid electrophoresis protocols. Bring final volume to 7.5 mL 0.1% (w/v) SDS. equipment at preferred prices. If you are an educator at the high school or college level, visit our Education Discount Policy page to establish an education account number. To choose the best gel for your application, see the gel selection guide. Add ethidium bromide to a final concentration of 0.5 µg/mL before pouring gel, Ampicillin 1000X See the sample label below for the location of this information. 57.1 mL glacial acetic acid 5.0 g ampicillin in H2O, 50 mL final volume (100 mg/mL final concentration) Agarose gel Make 1 mL aliquots, store at -20°C The lot number or control number (one or the other, not both) is printed on your product label. 270 mM KCl (20.1 g) 1 M Tris-HCl (for stacking gel) There are Certificates of Analysis that correspond to products that are either discontinued or simply not available on the website. Make 1 mL aliquots, store at -20°C, Kanamycin 1000X Your subscription information already exists, we will send you an email with specific instructions to manage your existing subscription profile. Please try reloading the page. Reduce preparation time and eliminate a source of error by using Bio-Rad’s range of premixed protein electrophoresis running buffers. A wide variety of premixed Tris buffers (such as Tris/glycine/SDS, Tris/glycine, and Tris/tricine/SDS) and high-purity, electrophoresis-grade powders (such as Tris, glycine, tricine, and urea) are available for protein electrophoresis and can be used with both handcast gels and Bio-Rad’s precast gels (see the protein gel selection guide).